An earlier strategy report to inform donor investments in international agricultural research highlighted the importance of animal-source foods in human nutrition in poorer countries as well as the strategic positioning of the livestock sector for meeting globally identified objectives for human development. That report presented a selection of intervention options with potential for improving outcomes in low-resource livestock production systems that centered on technological solutions to enduring challenges of animal nutrition and health, as well as options for securing the livestock assets of producers under adverse environmental and climate conditions. In this updated report, an expanded selection of technologies with promise for delivering food security, employment generation and related dividends in smallholder livestock settings is presented. The report details suggested approaches to the development and deployment of vaccines for key cattle and small ruminant diseases, advances addressing broader issues of animal health, associated human health and food safety. Focus is also placed on technologies related to animal nutrition and genetics and to the management of the natural resource base. Beyond scientific advancements, the report highlights policy and market changes, and other adjustments that are needed to address pressing constraints to livestock sector development in lower income countries. The full report was compiled following extensive consultations with scientists and managers at the ILRI and associates.